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Shenzhen Zhong Jian South Environment Co., Ltd.
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High Pressure Steam Sterilizers The Ultimate Solution for Ensuring Laboratory Safety and Reliability

Product Details

Place of Origin: China

Brand Name: ZJNF

Payment & Shipping Terms

Minimum Order Quantity: 1

Packaging Details: Plywood

Delivery Time: 20DAYS

Payment Terms: L/C, T/T, Western Union

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High Pressure Steam Sterilizers The Ultimate Solution for Ensuring Laboratory Safety and Reliability

Comprehensive Guide to Laboratory Autoclave (High-Pressure Steam Sterilizer)

I. Principles and Functions

Autoclaves use high-temperature, high-pressure saturated steam (typically 121°C, 0.1MPa) to denature microbial proteins, achieving sterilization‌This method is suitable for heat-resistant materials such as culture media, glassware, and surgical instruments, effectively eliminating all microorganisms, including bacterial spores‌


II. Classification and Structure

  1. Types

    • Portable‌: Compact (8L–30L), lightweight, ideal for routine laboratory use‌
    • Vertical‌: Larger capacity (30L–200L), designed for hospitals or bulk sterilization‌
    • Pre-vacuum‌: Removes air before sterilization to enhance steam penetration, suitable for porous or complex items‌
  2. Key Components

    • Sealed chamber, pressure gauge, safety valve, exhaust valve, and heating elements‌
    • Advanced models feature automated control systems (e.g., microprocessor-regulated pressure/temperature/time)‌

III. Operating Procedures

  1. Preparation

    • Verify water level, safety valve integrity, and chamber sealing‌
    • Load items into the inner basket without overcrowding to ensure steam circulation‌
  2. Sterilization Phase

    • Heat to generate steam, expel residual air via exhaust valve, then close the valve‌
    • Maintain target pressure (e.g., 0.1MPa) for 15–30 minutes (adjust based on material type)‌
  3. Completion

    • Allow natural cooling until pressure drops to zero. Open the exhaust valve and lid cautiously to avoid burns‌

IV. Safety and Maintenance

  1. Safety Protocols

    • Operator training is mandatory. ‌Never open the lid before the pressure has fully released‌‌
    • For liquid sterilization, vent pressure gradually to prevent boiling overflow‌
  2. Maintenance

    • Regularly inspect safety valves, seals, and exhaust lines for blockages or wear‌
    • Remove mineral deposits post-use to maintain heating efficiency‌

V. Typical Applications

  • Laboratories‌: Sterilizing culture media, lab tools‌
  • Medical Settings‌: Disinfecting surgical instruments, dressings‌
  • Food Industry‌: Packaging and canned goods sterilization‌
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